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KMID : 0613820070170030407
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Journal of Life Science
2007 Volume.17 No. 3 p.407 ~ p.411
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Regulation of β-xylosidase biosynthesis in Paenibacillus sp. DG-22
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Lee Tae-Hyeong
Lim Pyung-Ok
Lee Yong-Eok
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Abstract
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Regulation of β- xylosidase synthesis in Paenibacillus sp. DG-22 was studied to optimize the enzyme production. β-Xylosidase synthesis of the Paenibacillus sp. DG-22 was observed to be regulated by carbon sources present in culture media. The synthesis of β-xylosidase was induced by xylan and methyl β-D-xylopyranoside (βMeXyl) but slightly repressed by readily metabolizable monosaccharides. βMeXyl was found to be the best substrate for the induction of β-xylosidase and the most effective induction was obtained at a concentration of 10 mg/ml. β-Xylosidase production showed a cell growth associated profile with the maximum amount formed during the late exponential phase of growth. The presence of glucose and xylose decreased the level of β-xylosidase activity indicating that its production was subjected to a form of carbon catabolite repression. SDS-PAGE and zymogram techniques demonstrated the induction by βMeXyl and revealed the presence of one β-xylosidase of approximately 80 kDa.
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KEYWORD
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Paenibacillus sp, DG-22, β-xylosidase, regulation
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